preparation and in-vitro characterization of alginate microspheres incorporating leptospiral antigens as a delivery system and adjuvant

نویسندگان

f. inanlou

department of microbiology, faculty of basic sciences, islamic azad university, karaj branch, karaj, iran n. khaki

department of microbiology, razi vaccine and serum research institute, karaj, iran n. mohammadpour

department of venom and human sera, razi vaccine and serum research institute, karaj, iran h. zolfagharian

department of venom and human sera, razi vaccine and serum research institute, karaj, iran

چکیده

leptospirosis is one of the most prevalent zoonotic diseases worldwide. currently, multivalent whole-cell leptospiral vaccines can induce protection against leptospirosis. therefore, preparation and formulation of new generations of vaccines that can stimulate long-term immunity for leptospirosis control are essential. the aim of this study was to prepare and characterize alginate microspheres as an antigen delivery system for immunization against leptospirosis. we used five leptospira interrogans serovars, namely, icterohaemorrhagiae, grippotyphosa, serjo harjo, pomona, and canicola, for antigen preparation. alginate microspheres containing leptospiral antigen (la) were prepared by an emulsification method and evaluated with respect to morphology, size distribution, loading efficiency (le), loading capacity (lc), and release profile. the effects of concentration of alginate and emulsifiers and stirring rate on characteristics of microspheres were investigated. the optimal condition parameters for the preparation of la-loaded alginate microspheres were estimated. the optimum concentrations obtained for alginate and emulsifiers were 3.65% (w/v), span 80 (0.24% w/v), and tween 80 (3.85% w/v), respectively. moreover, the appropriate homogenization rate was 500 rpm. our results showed mean particle size of 200 μm, 97.41% le, and 8% lc for the microspheres. sufficient release profile was observed for in-vitro release test of la from alginate microspheres over an extended period of time (216 hour). therefore, alginate microspheres technologically seem to be a promising antigen delivery system for leptospiral vaccine.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Preparation and in-vitro characterization of alginate microspheres incorporating leptospiral antigens as a delivery system and adjuvant

Leptospirosis is one of the most prevalent zoonotic diseases worldwide. Currently, multivalent whole-cell leptospiral vaccines can induce protection against leptospirosis. Therefore, preparation and formulation of new generations of vaccines that can stimulate long-term immunity for leptospirosis control are essential. The aim of this study was to prepare and characterize alginate microspheres ...

متن کامل

Alginate Microsphere as a Delivery System and Adjuvant for Autoclaved Leishmania major and Quillaja Saponin: Preparation and Characterization

     The goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. Microspheres encapsulated with autoclaved Leishmania major (ALM) and Quillaja saponin (QS) were prepared by an emulsification technique and characterized for size, encapsulation efficiency and release profile of encapsulate...

متن کامل

Preparation and in-vitro evaluation of sodium alginate microspheres containing diphtheria toxoid as new vaccine delivery

  During last two decades, polysaccharides such as alginate (Alg) alone and in combination with other biopolymers are widely used in vaccine and drug delivery systems. The aim of the present work was to investigate the potential utility of microparticles made of alginate (Alg) as new vehicles for improving nasal vaccine delivery. For this purpose, diphtheria toxoid (DT) was chosen as a model an...

متن کامل

preparation and in-vitro evaluation of sodium alginate microspheres containing diphtheria toxoid as new vaccine delivery

during last two decades, polysaccharides such as alginate (alg) alone and in combination with other biopolymers are widely used in vaccine and drug delivery systems. the aim of the present work was to investigate the potential utility of microparticles made of alginate (alg) as new vehicles for improving nasal vaccine delivery. for this purpose, diphtheria toxoid (dt) was chosen as a model anti...

متن کامل

alginate microsphere as a delivery system and adjuvant for autoclaved leishmania major and quillaja saponin: preparation and characterization

the goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. microspheres encapsulated with autoclaved leishmania major (alm) and quillaja saponin (qs) were prepared by an emulsification technique and characterized for size, encapsulation efficiency and release profile of encapsulates. selection o...

متن کامل

Preparation and In Vitro Characterization of Alginate Microspheres Encapsulated with Autoclaved Leishmania major (ALM) and CpG -ODN

Objective The goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. Materials and Methods Microspheres were prepared by an emulsification technique and characterized for size, encapsulation efficiency, and release profile of encapsulates. Selection of appropriate parameters (viscosity of al...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
archives of razi institute

جلد ۷۱، شماره ۳، صفحات ۱۶۱-۱۶۸

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023